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2023

  • Guerra-Slompo EP, Cesaro G, Guimarães BG, Zanchin NIT. Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system. Nucleic Acids Res. 2023 Jan 11;51(1):396-419. doi: 10.1093/nar/gkac1217.

 

  • Cesaro G, da Soler HT, Guerra-Slompo EP, Haouz A, Legrand P, Zanchin NIT, Guimaraes BG. Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates. Nucleic Acids Res. 2023 Jan 11;51(1):380-395. doi: 10.1093/nar/gkac1199.

 

  • Guerra-Slompo EP, Picchi-Constante GFA, Marek M, Romier C, Sippl W, Zanchin NIT. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protoc. 2023 Jan 24;4(1):102058. doi: 10.1016/j.xpro.2023.102058.

 

  • Santos A, Macedo de Souza Brandão AP, Hryniewicz BM, Abreu H, Bach-Toledo L, Schuster da Silva S, Deller AE, Rogerio VZ, Baêta Rodrigues DS, Hiraiwa PM, Guimarães BG, Marchesi LF, Carvalho de Oliveira J, Gradia DF, Soares FLF, Zanchin NIT, Camargo de Oliveira C, Vidotti M. COVID-19 impedimetric biosensor based on polypyrrole nanotubes, nickel hydroxide and VHH antibody fragment: specific, sensitive, and rapid viral detection in saliva samples. Mater Today Chem. 2023 Jun;30:101597. doi: 10.1016/j.mtchem.2023.101597

 

2022

  • Fernandes LA, Gomes AA, Guimarães BG, de Lourdes Borba Magalhães M, Ray P, da Silva GF. Engineering defensin α-helix to produce high-affinity SARS-CoV-2 spike protein binding ligands. Protein Sci. 2022 Jun;31(6):e4355. doi: 10.1002/pro.4355.

 

Figura-de-Estrela-PNG-1024x973Picchi-Constante GFA, Hiraiwa PM, Marek M, Rogerio VZ, Guerra-Slompo EP, Romier C, Zanchin NIT. Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi. STAR Protoc. 2022 Apr 21;3(2):101324. doi: 10.1016/j.xpro.2022.101324.

 

Figura-de-Estrela-PNG-1024x973Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimarães BG, Zanchin NIT. A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9.

 

  • Dos Santos EF, Silva AAO, Freitas NEM, Leony LM, Daltro RT, Santos CAST, de Almeida MDCC, de Araújo FLV, Celedon PAF, Krieger MA, Zanchin NIT, Dos Reis MG, Santos FLN. Performance of Chimeric Trypanosoma cruzi Antigens in Serological Screening for Chagas Disease in Blood  Banks. Front Med (Lausanne). 2022 Mar 7;9:852864. doi: 10.3389/fmed.2022.852864.

 

Figura-de-Estrela-PNG-1024x973Freitas NEM, Santos EF, Leony LM, Silva ÂAO, Daltro RT, Vasconcelos LCM, Duarte GA, Oliveira da Mota C, Silva ED, Celedon PAF, Zanchin NIT, Santos FLN. Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera. PLoS Negl Trop Dis. 2022 Mar 11;16(3):e0010290. doi: 10.1371/journal.pntd.0010290.

 

  • Inoue AH, Domingues PF, Serpeloni M, Hiraiwa PM, Vidal NM, Butterfield ER, Del Pino RC, Ludwig A, Boehm C, Field MC, Ávila AR. Proteomics Uncovers Novel Components of an Interactive Protein Network Supporting RNA Export in Trypanosomes. Mol Cell Proteomics. 2022 Mar;21(3):100208. doi: 10.1016/j.mcpro.2022.100208.

 

  • Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Bueno NF, Guimarães BG, Zanchin NIT. Functional EGF domain of the human neuregulin 1α produced in Escherichia coli with accurate disulfide bonds. Mol Biol Rep. 2022 Dec;49(12):11715-11727. doi: 10.1007/s11033-022-07956-3.

 

  • Daltro RT, Santos EF, Silva ÂAO, Maron Freitas NE, Leony LM, Vasconcelos LCM, Luquetti AO, Celedon PAF, Zanchin NIT, Regis-Silva CG, Santos FLN. Western blot using Trypanosoma cruzi chimeric recombinant proteins for the serodiagnosis of chronic Chagas disease: A proof-of-concept study. PLoS Negl Trop Dis. 2022 Nov 28;16(11):e0010944. doi: 10.1371/journal.pntd.0010944.

 


 

2021

Figura-de-Estrela-PNG-1024x973Marek M, Ramos-Morales E, Picchi-Constante GFA, Bayer T, Norström C, Herp D, Sales-Junior PA, Guerra-Slompo EP, Hausmann K, Chakrabarti A, Shaik TB, Merz A, Troesch E, Schmidtkunz K, Goldenberg S, Pierce RJ, Mourão MM, Jung M, Schultz J, Sippl W, Zanchin NIT, Romier C. Species-selective targeting of pathogens revealed by the atypical structure and active site of Trypanosoma cruzi histone deacetylase DAC2. Cell Rep. 2021 Dec 21;37(12):110129. doi: 10.1016/j.celrep.2021.110129.

 

Figura-de-Estrela-PNG-1024x973de Lima GB, de Lima Cavalcanti TYV, de Brito ANALM, de Assis LA, Andrade-Vieira RP, Freire ER, da Silva Assunção TR, de Souza Reis CR, Zanchin NIT, Guimarães BG, de-Melo-Neto OP. The translation initiation factor EIF4E5 from Leishmania: crystal structure and interacting partners. RNA Biol. 2021 Dec;18(12):2433-2449. doi: 10.1080/15476286.2021.1918919.

 

Figura-de-Estrela-PNG-1024x973Picchi-Constante GFA, Guerra-Slompo EP, Tahira AC, Alcantara MV, Amaral MS, Ferreira AS, Batista M, Batista CM, Goldenberg S, Verjovski-Almeida S, Zanchin NIT. Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells. Sci Rep. 2021 Nov 4;11(1):21671. doi: 10.1038/s41598-021-01080-1.

 

  • Ambrosio AR, Bavia L, Hiraiwa PM, Tirado TC, Figueiredo FB, de Messias-Reason IJ. The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes. Life Sci. 2021 Oct 1;282:119793. doi: 10.1016/j.lfs.2021.119793.

 

  • Celedon PAF, Leony LM, Oliveira UD, Freitas NEM, Silva ÂAO, Daltro RT, Santos EF, Krieger MA, Zanchin NIT, Santos FLN. Stability Assessment of Four Chimeric Proteins for Human Chagas Disease Immunodiagnosis. Biosensors (Basel). 2021 Aug 22;11(8):289. doi: 10.3390/bios11080289.

 

  • Santos EF, Leony LM, Silva ÂAO, Daltro RT, Freitas NEM, Vasconcelos LCM, de Araújo FLV, Celedon PAF, Krieger MA, Zanchin NIT, Santos FLN. Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens. Transfusion. 2021 Sep;61(9):2701-2709. doi: 10.1111/trf.16583.

 

  • Guimarães BG, Golinelli-Pimpaneau B. De novo crystal structure determination of double stranded RNA binding domain using only the sulfur anomalous diffraction in SAD phasing. Curr Res Struct Biol. 2021 Jun 6;3:112-120. doi: 10.1016/j.crstbi.2021.05.002.

 

  • Chavas LMG, Gourhant P, Guimaraes BG, Isabet T, Legrand P, Lener R, Montaville P, Sirigu S, Thompson A. PROXIMA-1 beamline for macromolecular crystallography measurements at Synchrotron SOLEIL. J Synchrotron Radiat. 2021 May 1;28(Pt 3):970-976. doi: 10.1107/S1600577521002605.

 

Figura-de-Estrela-PNG-1024x973Oliveira UD, Santos FLN, Galvão-Castro B, Krieger MA, Zanchin NIT. Novel Genetic Constructs for Production of Recombinant HTLV-1/2 Antigens and Evaluation of Their Reactivity to Plasma Samples from HTLV-1-Infected Patients. J Clin Microbiol. 2021 Mar 19;59(4):e02701-20. doi: 10.1128/JCM.02701-20.

 

  • Bonilauri B, Santos MDM, Camillo-Andrade AC, Bispo S, Nogueira FCS, Carvalho PC, Zanchin NIT, Fischer JSDG. The impact of blood-processing time on the proteome of human peripheral blood mononuclear cells. Biochim Biophys Acta Proteins Proteom. 2021 Mar;1869(3):140581. doi: 10.1016/j.bbapap.2020.140581.

 


 

2020

  • Ambrosio AR, Bavia L, Borges BS, Hiraiwa PM, Pietsch JLM, Ribeiro MCVDC, Figueiredo FB, Messias-Reason IJ. Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensis. Acta Trop. 2020 Dec;212:105673. doi: 10.1016/j.actatropica.2020.105673.

 

  • Araujo LML, Souza C, Zanchin NIT, Rosário Filho NA. Identification of the major allergenic proteins from silkworm moth (Bombyx mori) involved in respiratory allergic diseases. Allergol Immunopathol (Madr). 2020 Nov-Dec;48(6):597-602. doi: 10.1016/j.aller.2019.12.003.

 

  • Pellizza L, López JL, Vázquez S, Sycz G, Guimarães BG, Rinaldi J, Goldbaum FA, Aran M, Mac Cormack WP, Klinke S. Structure of the putative long tail fiber receptor-binding tip of a novel temperate bacteriophage from the Antarctic bacterium Bizionia argentinensis JUB59. J Struct Biol. 2020 Oct 1;212(1):107595. doi: 10.1016/j.jsb.2020.107595.

 

  • Silva ED, Silva ÂAO, Santos EF, Leony LM, Freitas NEM, Daltro RT, Ferreira AGP, Diniz RL, Bernardo AR, Luquetti AO, Krieger MA, Celedon PAF, Viñas PA, Zanchin NIT, Santos FLN. Development of a New Lateral Flow Assay Based on IBMP-8.1 and IBMP-8.4 Chimeric Antigens to Diagnose Chagas Disease. Biomed Res Int. 2020 Aug 17;2020:1803515. doi: 10.1155/2020/1803515.

 

  • Gomes AA, da Silva GF, Lakkaraju SK, Guimarães BG, MacKerell AD Jr, Magalhães MLB. Insights into Glucose-6-phosphate Allosteric Activation of β-Glucosidase A. J Chem Inf Model. 2021 Apr 26;61(4):1931-1941. doi: 10.1021/acs.jcim.0c01450.

 

  • Resende BC, Oliveira ACS, Guañabens ACP, Repolês BM, Santana V, Hiraiwa PM, Pena SDJ, Franco GR, Macedo AM, Tahara EB, Fragoso SP, Andrade LO, Machado CR. The Influence of Recombinational Processes to Induce Dormancy in Trypanosoma cruzi. Front Cell Infect Microbiol. 2020 Jan 28;10:5. doi: 10.3389/fcimb.2020.00005.

 


 

2019

  • Camillo Dos Santos LP, de Matos BM, de Maman Ribeiro BC, Zanchin NIT, Guimarães BG. Crystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation factor EIF4G5. Acta Crystallogr F Struct Biol Commun. 2019 Dec 1;75(Pt 12):738-743. doi: 10.1107/S2053230X19015061.

 

Figura-de-Estrela-PNG-1024x973da Silva MS, Cayres-Silva GR, Vitarelli MO, Marin PA, Hiraiwa PM, Araújo CB, Scholl BB, Ávila AR, McCulloch R, Reis MS, Elias MC. Transcription activity contributes to the firing of non-constitutive origins in African trypanosomes helping to maintain robustness in S-phase duration. Sci Rep. 2019 Dec 6;9(1):18512. doi: 10.1038/s41598-019-54366-w.

 

  • Rodrigues EC, Mörking P, Rosa JO, Romagnoli BAA, Guimarães BG, Hiraiwa PM, Klinke A, de Aguiar AM, Kuligovski C, Goldenberg S, Ramos ASP. TcNST2 encodes a Golgi-localized UDP-galactose transporter in Trypanosoma cruzi. Parasitology. 2019 Sep;146(11):1379-1386. doi: 10.1017/S0031182019000738.

 

  • Daltro RT, Leony LM, Freitas NEM, Silva ÂAO, Santos EF, Del-Rei RP, Brito MEF, Brandão-Filho SP, Gomes YM, Silva MS, Donato ST, Jeronimo SMB, Monteiro GRG, Carvalho LP, Magalhães AS, Zanchin NIT, Celedon PAF, Santos FLN. Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic. J Clin Microbiol. 2019 Jul 26;57(8):e00762-19. doi: 10.1128/JCM.00762-19.

 

Figura-de-Estrela-PNG-1024x973Reolon LW, Vichier-Guerre S, de Matos BM, Dugué L, Assunção TRDS, Zanchin NIT, Pochet S, Guimarães BG. Crystal structure of the Trypanosoma cruzi EIF4E5 translation factor homologue in complex with mRNA cap-4. Nucleic Acids Res. 2019 Jun 20;47(11):5973-5987. doi: 10.1093/nar/gkz339.

 

  • Stein RJ, Duarte GL, Scheunemann L, Spohr MG, de Araújo Júnior AT, Ricachenevsky FK, Rosa LMG, Zanchin NIT, Dos Santos RP, Fett JP. Genotype Variation in Rice (Oryza sativa L.) Tolerance to Fe Toxicity Might Be Linked to Root Cell Wall Lignification. Front Plant Sci. 2019 Jun 12;10:746. doi: 10.3389/fpls.2019.00746.

 

  • Leony LM, Freitas NEM, Del-Rei RP, Carneiro CM, Reis AB, Jansen AM, Xavier SCC, Gomes YM, Silva ED, Reis MG, Fraga DBM, Celedon PAF, Zanchin NIT, Dantas-Torres F, Santos FLN. Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs. PLoS Negl Trop Dis. 2019 Jun 26;13(6):e0007545. doi: 10.1371/journal.pntd.0007545.

 

  • Del-Rei RP, Leony LM, Celedon PAF, Zanchin NIT, Reis MGD, Gomes YM, Schijman AG, Longhi SA, Santos FLN. Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries. PLoS One. 2019 Apr 18;14(4):e0215623. doi: 10.1371/journal.pone.0215623.

 

  • Dopico E, Del-Rei RP, Espinoza B, Ubillos I, Zanchin NIT, Sulleiro E, Moure Z, Celedon PAF, Souza WV, da Silva ED, Gomes YM, Santos FLN. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infect Dis. 2019 Mar 12;19(1):251. doi: 10.1186/s12879-019-3872-z.

 

Figura-de-Estrela-PNG-1024x973Dos Santos Rodrigues FH, Firczuk H, Breeze AL, Cameron AD, Walko M, Wilson AJ, Zanchin NIT, McCarthy JEG. The Leishmania PABP1-eIF4E4 interface: a novel 5′-3′ interaction architecture for trans-spliced mRNAs. Nucleic Acids Res. 2019 Feb 20;47(3):1493-1504. doi: 10.1093/nar/gky1187.

 

  • Faret M, de Morais SB, Zanchin NIT, de Souza TACB. L-Asparaginase from Erwinia carotovora: insights about its stability and activity. Mol Biol Rep. 2019 Feb;46(1):1313-1316. doi: 10.1007/s11033-018-4459-2.

 

Figura-de-Estrela-PNG-1024x973Cesaro G, Carneiro FRG, Ávila AR, Zanchin NIT, Guimarães BG. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biol. 2019 Jan;16(1):133-143. doi: 10.1080/15476286.2018.1564463.

 


 

2018

  • Santos FLN, Campos ACP, Amorim LDAF, Silva ED, Zanchin NIT, Celedon PAF, Del-Rei RP, Krieger MA, Gomes YM. Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis. Am J Trop Med Hyg. 2018 Nov;99(5):1174-1179. doi: 10.4269/ajtmh.17-0727.

 

  • Hiraiwa PM, de Aguiar AM, Ávila AR. Fluorescence-based assay for accurate measurement of transcriptional activity in trypanosomatid parasites. Cytometry A. 2018 Jul;93(7):727-736.

 

  • Alcantara MV, Kessler RL, Gonçalves REG, Marliére NP, Guarneri AA, Picchi GFA, Fragoso SP. Knockout of the CCCH zinc finger protein TcZC3H31 blocks Trypanosoma cruzi differentiation into the infective metacyclic form. Mol Biochem Parasitol. 2018 Apr;221:1-9.

 

  • Nunes HF, Ananina G, Costa VP, Zanchin NIT, de Vasconcellos JPC, de Melo MB. Investigation of CAV1/CAV2 rs4236601 and CDKN2B-AS1 rs2157719 in primary open-angle glaucoma patients from Brazil. Ophthalmic Genet. 2018 Apr;39(2):194-199.

 

  • Romagnoli BAA, Picchi GFA, Hiraiwa PM, Borges BS, Alves LR, Goldenberg S. Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi. Acta Trop. 2018 Feb;178:190-195.

 

  • Contreras Lancheros CA, Pelegrino MT, Kian D, Tavares ER, Hiraiwa PM, Goldenberg S, Nakamura CV, Yamauchi LM, Pinge-Filho P, Seabra AB, Yamada-Ogatta SF. Selective Antiprotozoal Activity of Nitric Oxide-releasing Chitosan Nanoparticles Against Trypanosoma cruzi: Toxicity and Mechanisms of Action. Curr Pharm Des. 2018;24(7):830-839.
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