Protein Purification and Biophysical Characterization

Facilities for expression of recombinants proteins in E. coli and yeast, purification and analysis of proteins in solution by circular dichroism, fluorescence and dynamic light scattering. Major equipment are: 4 chromatographers AKTA Purifier, a circular dichroism spectropolarimeter Jasco J-815, a fluorescence/luminescence spectrometer Perkin-Elmer LS-55, a dynamic light scattering DynaPro NanoStar and a Monolith NT.115 for microscale thermophoresis experiments.

Protein Crystallography

The protein crystallization laboratory includes an automated nanopipetor Mosquito (TTPLabTech) and an Olympus SZX-16 stereo microscope with a digital camera. In addition, computational resources for X-ray diffraction data processing and analysis of three-dimensional structures of biological macromolecules are available.

Our Laboratories